Heparin Medium

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Heparin Medium

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
Pancreatic digest of casein............................................................3.5g
NaCl..............................................................................................1.0g
Pancreatic digest of soybean meal................................................0.6g
Glucose .........................................................................................0.5g
K 2 HPO 4 .........................................................................................0.5g
Heparin solution.......................................................................10.0mL
pH 6.5 ± 0.2 at 25°C
Heparin Solution:
Composition per 10.0mL:
Heparin........................................................................................0.02g
Preparation of Heparin Solution: Add heparin to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except heparin solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile heparin solution. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation of Flavobacterium leparinum.