Herbaspirillum Agar

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Herbaspirillum Agar

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
KH 2 PO 4 .........................................................................................4.0g
MgSO 4 ·7H 2 O................................................................................0.2g
K 2 HPO 4 .........................................................................................0.1g
NaCl..............................................................................................0.1g
Yeast extract................................................................................0.05g
CaCl 2 ...........................................................................................0.02g
FeCl 2 ·6H 2 O.................................................................................0.01g
NaMoO 4 ·2H 2 O...........................................................................2.0mg
Solution A................................................................................50.0mL
pH 7.0 ± 0.2 at 25°C
Solution A:
Composition per 50.0mL
Sodium malate..............................................................................5.0g
Preparation of Solution A: Add sodium malate to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Adjust pH to 7.0. Filter sterilize.
Preparation of Medium: Add components, except solution A, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Aseptically add 50.0mL of sterile solution A. Mix thoroughly. Aseptically pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the cultivation and maintenance of Herbaspirillum seropedicae.