Herellea Agar

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Herellea Agar

培养基配方

Composition per liter:
Agar............................................................................................16.0g
Pancreatic digest of casein..........................................................15.0g
Lactose........................................................................................10.0g
Maltose .......................................................................................10.0g
Enzymatic digest of soybean meal ...............................................5.0g
NaCl..............................................................................................5.0g
Bile salts......................................................................................1.25g
Bromcresol Purple......................................................................0.02g
pH 6.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Diagnostic Systems.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the isolation, cultivation, and differentiation of Gram-negative nonfermentative and fermentative bacteria. It is especially recommended for the differentiation of Acinetobacter (Herellea) species from Neisseria gonorrhoeae in urethral or vaginal specimens. Fermentative bacteria appear as yellow colonies surrounded by yellow zones. Nonfermentative bacteria, such as Acinetobacter species, appear as pale lavender colonies.