Hershey’s Tris-Buffered Salts Medium

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

Hershey’s Tris-Buffered Salts Medium

培养基配方

Composition per liter:
Tris(hydroxymethyl)amino-
methane buffer (0.1M solution)............................................12.1g
NaCl..............................................................................................5.4g
KCl................................................................................................3.0g
NH 4 Cl ...........................................................................................1.1g
MgCl 2 ........................................................................................0.095g
KH 2 PO 4 .....................................................................................0.087g
Na 2 SO 4 ......................................................................................0.023g
CaCl 2 .........................................................................................0.011g
FeCl 3 ........................................................................................0.16mg
Glucose solution ....................................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose .........................................................................................2.0g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of a variety of heterotrophic microorganisms.