HESNW Medium

[所属分类:培养基配方] [发布时间:2021-4-2] [发布人: ] [阅读次数:] [返回]

HESNW Medium

培养基配方

Composition per 1011.0mL:
Natural seawater ...........................................................................1.0L
Enrichment solution.................................................................10.0mL
Vitamin solution.........................................................................1.0mL
Enrichment Solution:
Composition per liter:
NaNO 3 .......................................................................................4.667g
Na 2 SiO 3 ·9H 2 O...........................................................................3.000g
Sodium glycerophosphate.........................................................0.667g
EDTA·2H 2 O..............................................................................0.553g
H 3 BO 3 .......................................................................................0.380g
Fe(NH 4 ) 2 (SO 4 ) 2 ·6H 2 O ..............................................................0.234g
MnSO 4 ·4H 2 O............................................................................0.054g
FeCl 3 ·6H 2 O...............................................................................0.016g
ZnSO 4 ·7H 2 O..............................................................................7.3mg
CoSO 4 ·7H 2 O..............................................................................1.6mg
Preparation of Enrichment Solution: Add Na 2 SiO 3 ·9H 2 O to distilled/deionized water. Mix thoroughly. Neutralize Na 2 SiO 3 ·9H 2 O with 1N HCl. Add 500.0mL of distilled/deionized water. Mix thoroughly. Add remaining components and bring volume to 1.0L with distilled/deionized water. Mix thoroughly. Filter sterilize.
Vitamin Solution:
Composition per liter:
Thiamine.......................................................................................0.1g
Vitamin B 12 ................................................................................2.0mg
Biotin .........................................................................................1.0mg
Preparation of Vitamin Solution:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Filter sterilize.
Preparation of Medium: Allow natural seawater to age for 2 months. Filter sterilize. Aseptically add 10.0mL of sterile enrichment solution and 1.0mL of sterile vitamin solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Amphiprora hyalina, Chlamydomonas hedleyi, Chlamydomonas provasolii, Chlorella saccharophila, Chroomonas salina, Pavlova lutheri, and Trichosphaerium species.