Heterotrophic Medium H3P (DSMZ Medium 428)

[所属分类:培养基配方] [发布时间:2021-4-6] [发布人: ] [阅读次数:] [返回]

Heterotrophic Medium H3P

(DSMZ Medium 428)

培养基配方

Composition per 1010.0mL:
Solution B..............................................................................855.0mL
Solution A................................................................................50.0mL
Solution E ................................................................................50.0mL
Solution D................................................................................30.0mL
Solution C................................................................................20.0mL
Standard vitamin solution..........................................................5.0mL
Solution A:
Composition per 50.0mL:
Na 2 HPO 4 ·2H 2 O.............................................................................2.9g
KH 2 PO 4 .........................................................................................2.3g
Preparation of Solution A : Add components to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution B:
Composition per 855.0mL:
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCl 2 ·2H 2 O...............................................................................0.010g
MnCl 2 ·4H 2 O..............................................................................0.005g
NaVO 2 ·H 2 O ..............................................................................0.005g
Trace elements solution SL-6 ....................................................5.0mL
Preparation of Solution B : Add components to distilled/deionized water and bring volume to 855.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Trace Elements Solution SL-6:
Composition per liter:
MnCl 2 ·4H 2 O..................................................................................0.5g
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O................................................................................ 0.1g
Na 2 MoO 4 ·2H 2 O ......................................................................... 0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2 ·2H 2 O ................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to room temperature.
Solution C:
Composition per 20.0mL:
Ferric ammonium citrate.............................................................0.05g
Preparation of Solution C : Add ferric ammonium citrate to distilled/deionized water and bring volume to 20.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution D:
Composition per 30.0mL:
Yeast extract..................................................................................1.0g
Na-acetate .....................................................................................1.0g
Na 2 -succinate................................................................................1.0g
DL -Malate......................................................................................1.0g
Preparation of Solution D : Add components to distilled/deionized water and bring volume to 30.0mL. Mix thoroughly. Adjust to pH 7.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Solution E:
Composition per 50.0mL:
D -Glucose......................................................................................2.0g
Na-lactate......................................................................................1.0g
Na-pyruvate ..................................................................................1.0g
D -Mannitol....................................................................................1.0g
Preparation of Solution E : Add components to distilled/deionized water and bring volume to 50.0mL. Mix thoroughly. Adjust to pH 7.0. Filter sterilize.
Standard Vitamin Solution:
Composition per 100.0mL:
Thiamine-HCl·2H 2 O................................................................50.0mg
Nicotinic acid...........................................................................50.0mg
Pyridoxine-HCl........................................................................50.0mg
Ca-pantothenate.......................................................................50.0mg
Riboflavin................................................................................10.0mg
Vitamin B 12 ..............................................................................1.0mg
Folic acid ...................................................................................0.2mg
Biotin.........................................................................................0.1mg
Preparation of Standard Vitamin Solution : Add components to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Aseptically mix solutions A, B, C, D, E, and standard vitamin solution. Aseptically distribute to flasks or tubes.
Use:  For the cultivation and maintenance of Bacillus spp., Pseudomo- nas spp., Xanthomonas campestris pvar. campestris, Xanthobacter sp., Aquaspirillum arcticum, Herbaspirillum seropedicae, Sphingobium chlorophenolicum=Sphingomonas chlorophenolica, and Azoarcus sp. Also H3P is a heterotrophic medium for growth, purity checking, and isolation of a broad spectrum of aerobic bacteria.