Heterotrophic Medium for Hydrogenomonas

[所属分类:培养基配方] [发布时间:2021-4-6] [发布人: ] [阅读次数:] [返回]

Heterotrophic Medium for Hydrogenomonas

培养基配方

Composition per liter:
Agar............................................................................................15.0g
Tryptose........................................................................................5.0g
Cornstarch.....................................................................................2.0g
Sodium succinate·6H 2 O................................................................2.0g
Sodium glutamate.........................................................................1.0g
Yeast extract..................................................................................1.0g
Sodium citrate·2H 2 O.....................................................................0.5g
Sodium acetate·3H 2 O....................................................................0.3g
KH 2 PO 4 .........................................................................................0.2g
MgSO 4 ..........................................................................................0.1g
pH 6.8–7.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use:  For the heterotrophic cultivation of Hydrogenomonas species.