Heterotrophic Medium for Hydrogen-Oxidizing Bacteria

[所属分类:培养基配方] [发布时间:2021-4-6] [发布人: ] [阅读次数:] [返回]

Heterotrophic Medium for

Hydrogen-Oxidizing Bacteria

培养基配方

Composition per 1010.0mL:
Solution A..............................................................................900.0mL
Solution C..............................................................................100.0mL
Solution B................................................................................10.0mL
Solution A:
Composition per 900.0mL:
Noble agar...................................................................................17.0g
Na 2 HPO 4 ·12H 2 O...........................................................................9.0g
KH 2 PO 4 .........................................................................................1.5g
NH 4 Cl ...........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
Trace elements solution SL-6 ....................................................1.0mL
Preparation of Solution A: Add components to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Trace Elements Solution SL-6:
Composition per liter:
H 3 BO 3 ...........................................................................................0.3g
CoCl 2 ·6H 2 O ..................................................................................0.2g
ZnSO 4 ·7H 2 O.................................................................................0.1g
MnCl 2 ·4H 2 O................................................................................0.03g
Na 2 MoO 4 ·H 2 O ............................................................................0.03g
NiCl 2 ·6H 2 O.................................................................................0.02g
CuCl 2· 2H 2 O.................................................................................0.01g
Preparation of Trace Elements Solution SL-6: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 3.4.
Solution B:
Composition per 10.0mL:
CaCl 2 ·2H 2 O.................................................................................0.01g
Ferric ammonium citrate............................................................5.0mg
Preparation of Solution B: Add components to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Solution C:
Composition per 100.0mL:
Sodium 3-hydroxybutyrate...........................................................2.0g
Preparation of Solution C:  Add sodium 3-hydroxybutyrate to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize. Warm to 45°–50°C.
Preparation of Medium: Aseptically combine 900.0mL of sterile solution A, 10.0mL of sterile solution B, and 100.0mL of sterile solution C. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the heterotrophic cultivation and maintenance of Xanthobacter agilis.