Heterotrophic Nitrobacter Medium (DSMZ Medium 756)

[所属分类:培养基配方] [发布时间:2021-4-6] [发布人: ] [阅读次数:] [返回]

Heterotrophic Nitrobacter Medium

(DSMZ Medium 756)

培养基配方

Composition per liter:
Yeast extract..................................................................................1.5g
Peptone..........................................................................................1.5g
Na-pyruvate ................................................................................0.55g
Stock solution.........................................................................100.0mL
Trace elements solution .............................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Stock Solution:
Composition per liter:
NaCl..............................................................................................5.0g
KH 2 PO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCO 3 ........................................................................................0.07g
Preparation of Stock Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
FeSO 4 ·7H 2 O.............................................................................97.3mg
H 3 BO 3 ......................................................................................49.4mg
ZnSO 4 ·7H 2 O............................................................................43.1mg
(NH 4 ) 6 Mo 7 O 24 ·4H 2 O...............................................................37.1mg
MnSO 4 ·2H 2 O...........................................................................33.8mg
CuSO 4 ·5H 2 O............................................................................25.0mg

Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. 

Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use:  For the cultivation of Nitrospira moscoviensis, Nitrobacter hamburgensis, Nitrobacter vulgaris, and Nitrobacter winogradskyi.