Heterotrophic Nitrobacter Medium (LMG Medium 245)

[所属分类:培养基配方] [发布时间:2021-4-6] [发布人: ] [阅读次数:] [返回]

Heterotrophic Nitrobacter Medium

(LMG Medium 245)

培养基配方

Composition per liter:
Yeast extract..................................................................................1.5g
Peptone .........................................................................................1.5g
Na-pyruvate ................................................................................0.55g
Stock solution ........................................................................100.0mL
Trace elements solution.............................................................1.0mL
pH 7.4 ± 0.2 at 25°C
Stock Solution:
Composition per liter:
NaCl..............................................................................................5.0g
KH 2 PO 4 .........................................................................................1.5g
MgSO 4 ·7H 2 O................................................................................0.5g
CaCO 3 .........................................................................................0.07g
Preparation of Stock Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Trace Elements Solution:
Composition per liter:
(NH 4 )Mo7O 2 ..........................................................................437.1mg
FeSO 4 ·7H 2 O.............................................................................97.3mg
ZnSO 4 ·7H 2 O............................................................................43.1mg
H 3 BO 3 ......................................................................................39.4mg
MnSO 4 ·H 2 O.............................................................................33.8mg
CuSO 2 ·5H 2 O............................................................................25.0mg
Preparation of Trace Elements Solution: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4 with NaOH. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of heterotrophic Nitrobactger spp.