HiCrome™ Aureus Agar Base with Egg Tellurite (Aureus Agar Base, HiCrome™) (HiCrome™ Staphylococcus aureus Agar) (Staphylococcus aureus Agar, HiCrome)

[所属分类:培养基配方] [发布时间:2021-4-6] [发布人: ] [阅读次数:] [返回]

HiCrome™ Aureus Agar Base with Egg Tellurite
(Aureus Agar Base, HiCrome™)
(HiCrome™ Staphylococcus aureus Agar)

(Staphylococcus aureus Agar, HiCrome)

培养基配方

Composition per liter:
Agar ............................................................................................20.0g
Casein enzymic hydrolysate .......................................................12.0g
Sodium pyruvate.........................................................................10.0g
Beef extract...................................................................................6.0g
LiCl...............................................................................................5.0g
Yeast extract..................................................................................5.0g
Pancreatic digest of gelatin...........................................................3.0g
Chromogenic mixture...................................................................2.1g
Egg tellurite emulsion..............................................................50.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Caution: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapors. On contact with skin, wash with plenty of water immediately.
Egg Yolk Tellurite Emulsion:
Composition per 100.0mL:
Sterile saline.............................................................................64.0mL
Egg yolk...................................................................................30.0mL
Sterile potassium tellurite solution, 3.5%..................................6.0mL
Source: This medium is available from Fluka, Sigma-Aldrich, and HiMedia.
Preparation of Medium: Add components, except egg yolk tellu- rite emulsion, to distilled/deionized water and bring volume to 950.0mL. Mix thoroughly. Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 50.0mL sterile egg yok tellurite emulsion. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use: For the isolation and identification of staphylococci from environmental samples. For the isolation and enumeration of coagulase positive Staphylococcus aureus. Coagulase positive S. aureus gives brown-black colonies whereas S. epidermidis gives yellow, slightly brownish, colonies.