HiCrome™ Bacillus Agar wtih Polymyxin B (Bacillus cereus HiCrome™ Agar)

[所属分类:培养基配方] [发布时间:2021-4-6] [发布人: ] [阅读次数:] [返回]

HiCrome™ Bacillus Agar wtih Polymyxin B

(Bacillus cereus HiCrome™ Agar)

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
Peptic digest of animal tissue......................................................10.0g
D -Mannitol..................................................................................10.0g
NaCl............................................................................................10.0g
Chromogenic mixture...................................................................3.2g
Meat extract..................................................................................1.0g
Phenol Red................................................................................0.025g
Polymyxin B solution................................................................1.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium, without Polymyxin B solution, is available as a premixed powder from HiMedia.
Polymyxin B Solution:
Composition per 1.0mL:
Polymyxin B..............................................................................1.0mg
Preparation of Polymyxin B Solution: Add Polymyxin B to distilled/deionized water and bring volume to 1.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components, except Polymyxin B solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly and heat with frequent agitation until boiling. Boil until components are fully dissolved. Do not autoclave. Cool to 45°–50°C. Mix thoroughly. Aseptically add 1.0mL of Polymyxin B solution. Mix thoroughly. Pour into sterile Petri dishes.
Use: For the rapid identification of Bacillus species from a mixed cul- ture by chromogenic method. For the enumeration of Bacillus cereus and Bacillus thuringiensis when present in large numbers in certain foodstuffs.