HiCrome™ ECC Selective Agar Base, HiVeg

[所属分类:培养基配方] [发布时间:2021-4-7] [发布人: ] [阅读次数:] [返回]

HiCrome™ ECC Selective Agar Base, HiVeg

培养基配方

Composition per liter:
Agar ............................................................................................10.0g
Plant special peptone ....................................................................6.0g
Plant hydrolysate ..........................................................................3.3g
NaCl..............................................................................................2.0g
Sodium dihydrogen phosphate .....................................................0.6g
Na 2 HPO 4 .......................................................................................1.0g
Sodium pyruvate...........................................................................1.0g
Sorbitol .........................................................................................1.0g
Tryptophan....................................................................................1.0g
Chromogenic mixture.................................................................0.43g
Tergitol 7.....................................................................................0.15g
pH 7.0± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly and heat with frequent agitation in boiling water bath or flowing steam. Boil until components are fully dissolved (approximately 35 min). Do not autoclave. Cool to 45°–50°C. Pour into sterile Petri dishes. Medium may show haziness. To inhibit Pseudomonas and Aeromonas species 5 mg cefsulodin may be added for surface and pour plate methods or 10 mg cefsulodin may be aseptically added to the medium for membrane filter technique.
Use: For the detection of Escherichia coli and coliforms in water and food samples.