HiCrome™ ECD Agar with MUG

[所属分类:培养基配方] [发布时间:2021-4-7] [发布人: ] [阅读次数:] [返回]

HiCrome™ ECD Agar with MUG

培养基配方

Composition per liter:
Casein enzymic hydrolysate.......................................................20.0g
Agar............................................................................................15.0g
Lactose..........................................................................................5.0g
NaCl..............................................................................................5.0g
K 2 HPO 4 .........................................................................................4.0g
Bile salts mixture..........................................................................1.5g
KH 2 PO 4 .........................................................................................1.5g
L -Tryptophan ................................................................................1.0g
Chromogenic substrate .................................................................0.1g
Fluorogenic substrate..................................................................0.07g
pH 7.0 ± 0.2 at 37°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat while stirring and bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Pour into sterile Petri dishes. 

Use: For detection of Escherichia coli in a variety of specimens. Fluorescence in the UV and a positive indole test demonstrate the presence of E. coli in the colonies.