HiCrome™ Klebsiella Selective Agar Base with Carbenicillin

[所属分类:培养基配方] [发布时间:2021-4-8] [发布人: ] [阅读次数:] [返回]

HiCrome™ Klebsiella Selective Agar Base

with Carbenicillin

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
Peptone, special ..........................................................................12.0g
Yeast extract..................................................................................7.0g
NaCl..............................................................................................5.0g
Bile salts mixture..........................................................................1.5g
Chromogenic mixture...................................................................0.2g
Sodium lauryl sulfate....................................................................0.1g
Klebsiella selective supplement.................................................2.0mL
pH 7.1 ± 0.2 at 25°C
Source: This medium, without Klebsiella selective supplement, is available as a premixed powder from HiMedia.
Klebsiella Selective Supplement:
Composition per 2.0mL:
Carbenicillin.............................................................................50.0mg
Preparation of Klebsiella Selective Supplement: Add components to distilled/deionized water and bring volume to 2.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium:  Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly and heat with frequent agitation until boiling. Boil until components are fully dissolved. Do not autoclave. Cool to 45°–50°C. Aseptically add Klebsiella selective supplement. Mix well. Pour into sterile Petri dishes.
Use:  For the selective isolation and easy detection of Klebsiella species from water and sewage.