HiCrome™ Listeria Agar Base, Modified, with Moxalactam (Listeria HiCrome Agar Base, Modified)

[所属分类:培养基配方] [发布时间:2021-4-8] [发布人: ] [阅读次数:] [返回]

HiCrome™ Listeria Agar Base, Modified,
with Moxalactam

(Listeria HiCrome Agar Base, Modified)

培养基配方

Composition per liter:
Peptone, special ..........................................................................23.0g
Agar............................................................................................13.0g
Rhamnose ...................................................................................10.0g
Chromogenic mixture.................................................................5.13g
LiCl...............................................................................................5.0g
Meat extract..................................................................................5.0g
NaCl..............................................................................................5.0g
Yeast extract..................................................................................1.0g
Phenol Red..................................................................................0.12g
Moxolactam solution...............................................................10.0mL
pH 7.3 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Caution: Lithium chloride is harmful. Avoid bodily contact and inhalation of vapors. On contact with skin, wash with plenty of water immediately.
Moxalactam Solution (Listeria Selective Supplement):
Composition per 10.0mL:
Moxolactam..................................................................................0.2g
Preparation of Moxalactam Solution (Listeria Selective Supplement): Add moxalactam to distilled/deionized water and bring volume to 10.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except moxalactam solution, to distilled/deionized water and bring volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°– 50°C. Add 10.0mL moxalactam solution (Listeria selective supplement). Mix well. Pour into sterile Petri dishes.
Use: For the rapid and direct identification of Listeria species, specif- ically Listeria monocytogenes. A selective and differential agar medium recommended for rapid and direct identification of Listeria species, specifically Listeria monocytogenes.