Hirschia Medium

[所属分类:培养基配方] [发布时间:2021-4-9] [发布人: ] [阅读次数:] [返回]

Hirschia Medium

培养基配方

Composition per liter:
Pancreatic digest of casein............................................................5.0g
HEPES..........................................................................................4.0g
Yeast extract..................................................................................2.0g
Artificial seawater..................................................................250.0mL
Glucose solution....................................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
Glucose.......................................................................................0.25g
Preparation of Glucose Solution: Add glucose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Artificial Seawater:
Composition per liter:
NaCl............................................................................................27.5g
MgCl 2 ·6H 2 O ...............................................................................5.38g
MgSO 4 ·7H 2 O..............................................................................6.78g
KCl..............................................................................................0.72g
NaHCO 3 ....................................................................................... 0.2g
CaCL 2 ·2H 2 O.................................................................................1.4g
Preparation of Artificial Seawater: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except glucose solution, to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.4. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 100.0mL of sterile glucose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Hirschia baltica.