Hi-Sensitivity Test Agar

[所属分类:培养基配方] [发布时间:2021-4-9] [发布人: ] [阅读次数:] [返回]

Hi-Sensitivity Test Agar

培养基配方

Composition per liter:
Casein enzymic hydrolysate....................................................... 11.0g
Agar..............................................................................................8.0g
NaCl..............................................................................................3.0g
Peptic digest of animal tissue .......................................................3.0g
Glucose.........................................................................................2.0g
Na 2 HPO 4 .......................................................................................2.0g
Sodium acetate..............................................................................1.0g
Starch, soluble...............................................................................1.0g
Magnesium glycerophosphate......................................................0.2g
Calcium gluconate........................................................................0.1g
L -Cystine hydrochloride .............................................................0.02g
L -Tryptophan ..............................................................................0.02g
Adenine.......................................................................................0.01g
Guanine.......................................................................................0.01g
Uracil ..........................................................................................0.01g
Xanthine......................................................................................0.01g
Calcium pantothenate ................................................................3.0mg
Biotin.........................................................................................3.0mg
Nicotinamide..............................................................................3.0mg
Pyridoxine hydrochloride ..........................................................3.0mg
Manganese chloride...................................................................2.0mg
ZnSO 4 ........................................................................................1.0mg
CoSO 4 ........................................................................................1.0mg
CuSO 4 ........................................................................................1.0mg
Cyanocobalamin........................................................................1.0mg
FeSO 4 .........................................................................................1.0mg
Menadione .................................................................................1.0mg
Thiamine hydrochloride...........................................................0.04mg
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMedia.
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes.
Use:  For antimicrobial susceptibility tests.