HM Medium

[所属分类:培养基配方] [发布时间:2021-4-12] [发布人: ] [阅读次数:] [返回]

HM Medium

培养基配方

Composition per liter:
NaCl............................................................................................81.0g
Yeast extract................................................................................10.0g
MgSO 4 ..........................................................................................9.6g
MgCl 2 ............................................................................................7.0g
Proteose peptone No. 3.................................................................5.0g
KCl................................................................................................2.0g
Glucose.........................................................................................1.0g
CaCl 2 ...........................................................................................0.36g
NaHCO 3 ...................................................................................60.0mg
NaBr.........................................................................................26.0mg
pH 7.1 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 7.1. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C.
Use: For the cultivation of Salinicoccus roseus and Salinicoccus hispanicus.