HNS Agar (ATCC Medium 923)

[所属分类:培养基配方] [发布时间:2021-4-12] [发布人: ] [阅读次数:] [返回]

HNS Agar

(ATCC Medium 923)

培养基配方

Composition per liter:
Agar............................................................................................15.0g
NaCl..............................................................................................9.6g
Heart infusion broth...............................................................990.0mL
Horse serum.............................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Heart Infusion Broth:
Composition per 900.0mL:
Beef heart, infusion from..........................................................500.0g
Tryptose......................................................................................10.0g
NaCl..............................................................................................5.0g
Preparation of Heart Infusion Broth:  Add agar and NaCl to 990.0mL heart infusion broth. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add 10.0mL sterile horse serum. Mix thoroughly. Pour into sterile Petri dishes or distribute into sterile tubes.
Use:  For the cultivation and maintenance of Corynebacterium species.