Horie Arabinose Ethyl Violet Broth (HAEB)

[所属分类:培养基配方] [发布时间:2021-4-12] [发布人: ] [阅读次数:] [返回]

Horie Arabinose Ethyl Violet Broth

(HAEB)

培养基配方

Composition per liter:
NaCl............................................................................................30.0g
Peptone..........................................................................................5.0g
Beef extract...................................................................................3.0g
Bromthymol Blue .......................................................................0.03g
Ethyl Violet................................................................................1.0mg
Arabinose solution.................................................................100.0mL
pH 9.0 ± 0.2 at 25°C
Arabinose Solution:
Composition per 100.0mL:
Arabinose......................................................................................5.0g
Preparation of Arabinose Solution: Add arabinose to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except arabinose solution, to distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling. Adjust pH to 9.0. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Aseptically add sterile arabinose solution. Mix thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation of Vibrio species from foods.