Horikoshi Alkaline Medium (DSMZ Medium 940)

[所属分类:培养基配方] [发布时间:2021-4-12] [发布人: ] [阅读次数:] [返回]

Horikoshi Alkaline Medium

(DSMZ Medium 940)

培养基配方

Composition per liter:
Agar ............................................................................................15.0g
D -glucose.....................................................................................10.0g
Peptone..........................................................................................5.0g
Yeast extract..................................................................................5.0g
Na 2 CO 3 .........................................................................................5.0g
KH 2 PO 4 .........................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.2g
pH 9.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Pannonibacter phragmitetus.