Horikoshi-1 Medium (DSMZ Medium 1081)

[所属分类:培养基配方] [发布时间:2021-4-12] [发布人: ] [阅读次数:] [返回]

Horikoshi-1 Medium

(DSMZ Medium 1081)

培养基配方

Composition per liter:
Agar............................................................................................15.0g
Glucose ......................................................................................10.0g
Polypeptone ..................................................................................5.0g
Yeast extract .................................................................................5.0g
KH 2 PO 4 ........................................................................................1.0g
K 2 HPO 4 ........................................................................................1.0g
MgSO 4 · 7H 2 O................................................................................0.2g
NaCO 3 solution.......................................................................100.0mL
pH 10.0 ± 0.2 at 25°C
NaCO 3 Solution :
Composition per 100.0mL:
NaCO 3 .........................................................................................10.0g
Preparation of NaCO 3 Solution:  Add NaCO 3 to distilled/deionized water and bring volume to 100.0mL. Mix thoroughly. Filter sterilize.
Preparation of Medium: Add components, except NaCO 3 solution, to double distilled/deionized water and bring volume to 900.0mL. Mix thoroughly. Adjust pH to 10.0. Gently heat while stirring and bring to boiling. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically add 100.0mL NaCO 3 solution. Adjust pH to 10.0. Pour into Petri dishes or aseptically distribute into tubes.
Use: For the cultivation of “Streptomyces sannurensis” and Salinicoccus alkaliphilus.