Hottinger Broth

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

Hottinger Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Fish peptone................................................................................20.0g
Yeast extract..................................................................................2.0g
Tryptophan....................................................................................1.0g
pH 7.2 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from Hi-
Media.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use:  For cultivation of less fastidious microorganisms and determina-
tion of indole as per USSR State Pharmacopoeia.