Howardella Medium (DSMZ Medium 1085)

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

Howardella Medium
(DSMZ Medium 1085)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:
Casitone .....................................................................................20.0g
Yeast extract .................................................................................5.0g
Na 2 HPO 4 ......................................................................................5.0g
MgCl 2 ·6H 2 O .................................................................................1.1g
Urea ..............................................................................................1.0g
Na-thioglycolate ........................................................................0.75g
Resazurin ..................................................................................0.5mg
Urea solution............................................................................10.0mL
pH 7.4 ± 0.2 at 25°C
Urea Solution :
Composition per 10.0mL:
Urea ..............................................................................................1.0g
Preparation of Urea Solution:  Add urea to distilled/deionized wa-
ter and bring volume to 10.0mL. Mix thoroughly. Sparge with 100%
N 2 . Filter sterilize.
Preparation of Medium: Add components, except thioglycolate
and urea solution, to double distilled/deionized water and bring volume
to 990.0mL. Cool to room temperature while sparging with 80% N 2 +
20% CO 2 . Add thioglycolate. Mix thoroughly. Distribute into tubes or
bottles under an atmosphere of 80% N 2 + 20% CO 2 . Autoclave for 15
min at 15 psi pressure–121°C. Cool to room temperature. Aseptically
add 10.0mL urea solution. Adjust pH to 7.4.
Use: For the cultivation of Howardella spp.