Hoyer’s Medium

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

Hoyer’s Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

(NH 4 ) 2 SO 4 ....................................................................................1.0g
KH 2 PO 4 .........................................................................................0.9g
MgSO 4 ·7H 2 O..............................................................................0.25g
K 2 HPO 4 .........................................................................................0.1g
FeCl 3 ·6H 2 O.................................................................................0.02g
Ethanol solution.....................................................................200.0mL
Ethanol Solution:
Composition per 200.0mL:
Ethanol.....................................................................................30.0mL
Preparation of Ethanol Solution: Add ethanol to distilled/deion-
ized water and bring volume to 200.0mL. Mix thoroughly. Filter ster-

ilize.

Preparation of Medium: Add components, except ethanol solu-
tion, to distilled/deionized water and bring volume to 800.0mL. Mix
thoroughly. Distribute into tubes in 4.0mL volumes. Autoclave for 15
min at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 1.0mL of
sterile ethanol solution to each tube. Mix thoroughly.
Use: For the cultivation of Acetobacter species.