Hoyle Medium

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

Hoyle Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 1060.0mL:
Agar ............................................................................................15.0g
Lab Lemco powder.....................................................................10.0g
Peptone........................................................................................10.0g
NaCl..............................................................................................5.0g
Horse blood, laked...................................................................50.0mL
Tellurite solution......................................................................10.0mL
pH 7.8 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from Oxoid
Unipath.
Horse Blood, Laked:
Composition per 50.0mL:
Horse blood, fresh....................................................................50.0mL
Preparation of Horse Blood, Laked: Add blood to a sterile poly-
propylene bottle. Freeze overnight at −20°C. Thaw at 8°C. Refreeze at
−20°C. Thaw again at 8°C.
Tellurite Solution:
Composition per 100.0mL:
K 2 TeO 3 ..........................................................................................3.5g
Preparation of Tellurite Solution: Add K 2 TeO 3 to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Filter
sterilize.
Caution: Potassium tellurite is toxic.
Preparation of Medium: Add components, except laked horse
blood and tellurite solution, to distilled/deionized water and bring vol-
ume to 1.0L. Mix thoroughly. Gently heat and bring to boiling. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 50°C. Aseptically
add 50.0mL sterile laked horse blood and 10.0mL sterile tellurite solu-
tion. Mix thoroughly. Pour into sterile Petri dishes or distribute into ster-
ile tubes.
Use:  For the isolation and differentiation of Corynebacterium diphthe-
riae strains. This medium permits very rapid growth of all types of
Corynebacterium diphtheriae, so that diagnosis is possible after 18
hours’ incubation.