HP 6 Agar Base

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

HP 6 Agar Base

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Sodium glutaminate....................................................................10.0g
MgSO 4 ·7H 2 O................................................................................1.0g
Yeast extract..................................................................................1.0g
Cyanocobalamin ........................................................................0.5mg
Glucose solution ....................................................................100.0mL
Source: This medium is available from HiMedia.
Glucose Solution:
Composition per 100.0mL:
Glucose .........................................................................................5.0g
Preparation of Glucose Solution:  Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Preparation of Medium: Add components, except glucose solu-
tion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add glucose solution. Mix thoroughly. Pour into Pe-
tri dishes or aseptically distribute into sterile tubes.
Use: For the isolation and cultivation of Cytophaga, Herpetosiphon,
Saprospira, and Flexithrix species.