HP 74 Broth

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

HP 74 Broth

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Sodium glutaminate....................................................................10.0g
MgSO 4 ·7H 2 O................................................................................2.0g
Yeast extract..................................................................................2.0g
Glucose solution....................................................................100.0mL
Phosphate buffer solution........................................................20.0mL
pH 6.5 ± 0.2 at 25°C
Glucose Solution:
Composition per 100.0mL:
D- Glucose....................................................................................10.0g
Preparation of Glucose Solution: Add  D- glucose to distilled/de-
ionized water and bring volume to 100.0mL. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 25°C.
Phosphate Buffer Solution:
Composition per 100.0mL:
K 2 HPO 4 .......................................................................................6.81g
Preparation of Phosphate Buffer Solution: Add K 2 HPO 4 to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Adjust pH to 6.5. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 25°C.
Preparation of Medium: Add components, except glucose solu-
tion and phosphate buffer solution, to distilled/deionized water and
bring volume to 880.0mL. Mix thoroughly. Gently heat and bring to
boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–
50°C. Aseptically add 100.0mL of sterile glucose solution and 20.0mL
of sterile phosphate buffer solution. Mix thoroughly. Aseptically dis-
tribute into sterile tubes or flasks.
Use: For the isolation and cultivation of Cytophaga species, Herpeto-
siphon species, Saprospira species, and Flexithrix species.