HP Medium

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

HP Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of soybean meal..............................................20.0g
Beef extract.................................................................................10.0g
Yeast extract..................................................................................6.0g
Ammonium citrate........................................................................5.0g
Tween™ 80...................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
MnSO 4 ·4H 2 O..............................................................................0.05g
FeSO 4 ·7H 2 O................................................................................0.04g
Glucose solution ......................................................................10.0mL
Tetracycline solution................................................................10.0mL
Glucose Solution:
Composition per 100.0mL:
Glucose .......................................................................................10.0g
Preparation of Glucose Solution: Add glucose to distilled/deion-
ized water and bring volume to 100.0mL. Mix thoroughly. Filter ster-
ilize.
Tetracycline Solution:
Composition per 100.0mL:
Tetracycline.................................................................................10.0g
Preparation of Tetracycline Solution: Add tetracycline to dis-
tilled/deionized water and bring volume to 100.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components, except glucose solu-
tion and tetracycline solution, to distilled/deionized water and bring
volume to 990.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add sterile glucose solution and tetracycline solution. Mix
thoroughly. Aseptically distribute into sterile tubes or flasks.
Use: For the cultivation and enumeration of Leuconostoc species.