HPC Agar (Heterotrophic Plate Count Agar) (m-HPC Agar)

[所属分类:培养基配方] [发布时间:2021-4-16] [发布人:李佳颐] [阅读次数:] [返回]

HPC Agar
(Heterotrophic Plate Count Agar)
(m-HPC Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Gelatin.........................................................................................25.0g
Pancreatic digest of gelatin.........................................................20.0g
Agar ............................................................................................15.0g
Glycerol ...................................................................................10.0mL
pH 7.1 ± 0.2 at 25°C
Source:  This medium is available from BD Diagnostic Systems.
Preparation of Medium: Add components, except glycerol, to dis-
tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Add glycerol. Mix thoroughly. Auto-
clave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C. Pour into
sterile Petri dishes.
Use: For the the cultivation and enumeration of microorganisms from
potable water sources, swimming pools, and other water specimens by
the membrane filter method and heterotrophic plate count technique.