Lactic HiVeg Agar

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

Lactic HiVeg Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Plant hydrolysate.........................................................................22.5g
Agar ............................................................................................15.0g
Glucose .........................................................................................5.0g
Lactose..........................................................................................5.0g
Sucrose..........................................................................................5.0g
Yeast extract..................................................................................5.0g
NaCl..............................................................................................4.0g
Sodium acetate..............................................................................1.5g
Ascorbic acid ................................................................................0.5g
pH 6.0 ± 0.2 at 25°C
Source: This medium is available as a premixed powder from HiMe-
dia.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust pH to 5.3–5.4.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C.
Use: For the enumeration and identification of lactic streptococci and
lactobacilli by the pour plate method.