Lactic Streak Agar

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

Lactic Streak Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
Sodium carboxymethylcellulose.................................................10.0g
Calcium citrate............................................................................10.0g
Beef extract...................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
Polypeptone™...............................................................................5.0g
Yeast extract..................................................................................5.0g
Lactose..........................................................................................1.5g
L- Arginine·HCl..............................................................................1.5g
Bromcresol Purple (0.1% solution) .........................................20.0mL
pH 6.0 ± 0.1 at 25°C
Preparation of Medium: Add components—except Bromcresol
Purple solution, calcium citrate, and sodium carboxymethylcellu-
lose—to distilled/deionized water and bring volume to 800.0mL. Mix
thoroughly. Gently heat and bring to boiling. In a blender, add the cal-
cium citrate and sodium carboxymethylcellulose to 200.0mL of dis-
tilled/deionized water. Blend until mixed. Add the 200.0mL of citrate/
carboxymethylcellulose solution to the hot agar solution. Mix thor-
oughly. Adjust pH to 6.0. Distribute into flasks in 100.0mL volumes.
Autoclave for 10 min at 10 psi pressure–115°C. Cool to 45°–50°C.
Aseptically add 2.0mL of sterile Bromcresol Purple to each flask. Mix
thoroughly. Pour into sterile Petri dishes. Allow plates to dry at 37°C
for 1 hr before use.
Use: For the differentiation of lactic streptococci. Bacteria that pro-
duce acid from lactose appear as yellow colonies.