Lactobacilli MRS Broth (Lactobacilli deMan-Rogosa-Sharpe Broth)

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacilli MRS Broth
(Lactobacilli deMan-Rogosa-Sharpe Broth)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Beef extract.................................................................................10.0g
Peptone .......................................................................................10.0g
Sodium acetate..............................................................................5.0g
Yeast extract..................................................................................5.0g
Ammonium citrate........................................................................2.0g
Na 2 HPO 4 .......................................................................................2.0g
Tween™ 80...................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
MnSO 4 ·5H 2 O..............................................................................0.05g
pH 6.5 ± 0.2 at 25°C
Source:  This medium is available as a premixed powder from BD Di-
agnostic Systems.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the cultivation of Lactobacillus species. Also used for the
cultivation and maintenance of Aerococcus viridans, Bifidobacterium
coryneforme, Lactococcus plantarum, Leuconostoc species, Pectina-
tus cerevisiiphilus, Pediococcus species, and Sporolactobacillus inuli-
nus.