Lactobacilli MRS Broth with Cysteine

[所属分类:培养基配方] [发布时间:2021-4-26] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacilli MRS Broth with Cysteine

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................20.0g
Beef extract.................................................................................10.0g
Peptone .......................................................................................10.0g
Sodium acetate..............................................................................5.0g
Yeast extract..................................................................................5.0g
L -Cysteine.....................................................................................2.0g
Ammonium citrate........................................................................2.0g
Na 2 HPO 4 .......................................................................................2.0g
Tween™ 80...................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
MnSO 4 ·5H 2 O..............................................................................0.05g
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C.
Use: For the cultivation and maintenance of Lactobacillus species,
including L. ruminis and L. vitulinus.