Lactobacilli MRS Broth with Ethanol

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacilli MRS Broth with Ethanol

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Beef extract.................................................................................10.0g
Peptone........................................................................................10.0g
Sodium acetate..............................................................................5.0g
Yeast extract..................................................................................5.0g
L -Cysteine.....................................................................................2.0g
Ammonium citrate........................................................................2.0g
Na 2 HPO 4 .......................................................................................2.0g
Tween™ 80...................................................................................1.0g
MgSO 4 ·7H 2 O................................................................................0.1g
MnSO 4 ·5H 2 O..............................................................................0.05g
Ethanol (95% solution)..........................................................100.0mL
pH5.0 ± 0.2 at 25°C
Preparation of Medium: Add components, except ethanol, to dis-
tilled/deionized water and bring volume to 990.0mL. Mix thoroughly.
Gently heat and bring to boiling. Autoclave for 15 min at 15 psi pres-
sure–121°C. Cool to 25°C. Filter sterilize ethanol. Aseptically add
100.0mL of sterile ethanol. Mix thoroughly. Aseptically distribute into
sterile tubes or flasks.
Use: For the cultivation and maintenance of Lactobacillus species.