Lactobacillus Agar 2

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus Agar 2

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Sodium acetate............................................................................20.0g
Agar ............................................................................................15.0g
Tryptic digest of casein...............................................................10.0g
Yeast extract..................................................................................5.0g
Meat extract ..................................................................................2.0g
K 2 HPO 4 .........................................................................................0.5g
KH 2 PO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O.........................................................................200.0mg
DL -Mevalonic acid...................................................................30.0mg
FeSO 4 ·7H 2 O.............................................................................10.0mg
MnSO 4 ·H 2 O.............................................................................. 7.5mg
Ethanol.....................................................................................40.0mL
Tween™ 80................................................................................1.0mL
pH 5.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Lactobacillus fructiv-
orans and Lactobacillus homohiochii.