Lactobacillus bifidus Medium

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus bifidus Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Lactose........................................................................................70.0g
Sodium acetate, anhydrous.........................................................50.0g
Pancreatic digest of casein..........................................................10.0g
K 2 HPO 4 .........................................................................................5.0g
Tween™ 80...................................................................................1.0g
Alanine..........................................................................................0.4g
L -Cystine.......................................................................................0.4g
Tryptophan....................................................................................0.4g
Asparagine....................................................................................0.2g
Adenine.......................................................................................0.02g
Guanine.......................................................................................0.02g
Uracil..........................................................................................0.02g
Xanthine......................................................................................0.02g
Pyridoxine·HCl..........................................................................2.4mg
Nicotinic acid.............................................................................1.2mg
Calcium pantothenate ................................................................0.8mg
Riboflavin..................................................................................0.4mg
Thiamine·HCl............................................................................0.4mg
p-Aminobenzoic acid...............................................................0.02mg
Folic acid .................................................................................0.02mg
Biotin..........................................................................................8.0μg
Ascorbic acid solution ...........................................................100.0mL
Human milk, skimmed.............................................................20.0mL
Salts B......................................................................................10.0mL
pH 6.8 ± 0.2 at 25°C
Salts B:
Composition per 250.0mL:
MgSO 4 ·7H 2 O..............................................................................10.0g
FeSO 4 ·7H 2 O..................................................................................0.5g
NaCl..............................................................................................0.5g
MnSO 4 ·2H 2 O............................................................................0.337g
Preparation of Salts B: Add components to distilled/deionized wa-
ter and bring volume to 250.0mL. Mix thoroughly.
Ascorbic Acid Solution:
Composition per 100.0mL:
Ascorbic acid................................................................................1.0g
Preparation of Ascorbic Acid Solution: Add ascorbic acid to
distilled/deionized water and bring volume to 100.0mL. Mix thorough-
ly. Adjust pH to 6.5. Filter sterilize.
Preparation of Medium: Add components, except ascorbic acid
solution and human milk, to distilled/deionized water and bring vol-
ume to 900.0mL. Mix thoroughly. Gently heat and bring to boiling.
Autoclave for 15 min at 15 psi pressure–121°C. Cool to 45°–50°C.
Aseptically add 100.0mL of sterile ascorbic acid solution. Mix thor-
oughly. This constitutes a double-strength medium. To prepare a sin-
gle-strength medium, aseptically combine 500.0mL of sterile double-
strength medium, 480.0mL of sterile distilled/deionized water, and
20.0mL of sterile human milk. Aseptically distribute into sterile tubes
or flasks.
Use:  For the cultivation and maintenance of Bifidobacterium (Lacto-
bacillus) bifidum.