Lactobacillus bulgaricus Agar (LB Agar)

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus bulgaricus Agar
(LB Agar)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 900.0mL:

Agar............................................................................................20.0g
Glucose.......................................................................................20.0g
Beef extract.................................................................................10.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
K 2 HPO 4 .........................................................................................2.0g
Tween™ 80...................................................................................1.0g
Acetate buffer...........................................................................80.0mL
Tomato juice, filtered...............................................................40.0mL
pH 6.8 ± 0.2 at 25°C
Acetate Buffer:
Composition per liter:
Sodium acetate........................................................................113.55g
Acetic acid ...............................................................................10.0mL
Preparation of Acetate Buffer: Add components to distilled/de-
ionized water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Add components, except acetate buffer,
to distilled/deionized water and bring volume to 920.0mL. Mix thor-
oughly. Gently heat and bring to boiling. Adjust pH to 6.8. Add
80.0mL of acetate buffer. Mix thoroughly. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes.
Use: For the isolation, cultivation, and enumeration of Lactobacillus
bulgaricus from foods.