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Lactobacillus Medium (ATCC Medium 78)

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus Medium
(ATCC Medium 78)
山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein..........................................................20.0g
Agar............................................................................................15.0g
Tryptose........................................................................................5.0g
Yeast extract..................................................................................5.0g
Glucose.........................................................................................3.0g
Lactose..........................................................................................2.0g
Liver extract concentrate ..............................................................1.0g
Tween™ 80.................................................................................0.05g
Tomato juice, filtered.............................................................200.0mL
pH 6.5 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 6.5. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation and maintenance of Lactobacillus fermentum
and Lactobacillus salivarius.

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Lactobacillus Medium (ATCC Medium 78)