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Lactobacillus Medium (ATCC Medium 169)

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus Medium
(ATCC Medium 169)
山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose.......................................................................................10.0g
Proteose peptone...........................................................................7.5g
Yeast extract..................................................................................7.5g
KH 2 PO 4 .........................................................................................2.0g
L- Cysteine·HCl·H 2 O .....................................................................1.0g
Tween™ 80...................................................................................0.1g
Tomato juice, filtered.............................................................100.0mL
pH 7.0 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Adjust pH to 7.0. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the cultivation and maintenance of Lactobacillus casei.

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Lactobacillus Medium (ATCC Medium 169)