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Lactobacillus Medium

[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]

Lactobacillus Medium
山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .......................................................................................20.0g
Sodium acetate............................................................................20.0g
Tryptone......................................................................................10.0g
Yeast extract..................................................................................5.0g
Beef extract...................................................................................2.0g
K 2 HPO 4 ........................................................................................0.5g
KH 2 PO 4 ........................................................................................0.5g
MgSO 4 ·7H 2 O.........................................................................200.0mg
FeSO 4 ·7H 2 O ............................................................................10.0mg
MnSO 4 ·H 2 O...............................................................................7.5mg
Ethanol.....................................................................................40.0mL
Mevalonic acid solution...........................................................10.0mL
Tween™ 80................................................................................1.0mL
pH 5.2 ± 0.2 at 25°C
Mevalonic Acid Solution:
Composition per 10.0mL:
DL -Mevalonic acid...................................................................30.0mg
Preparation of Mevalonic Acid Solution: Add DL -mevalonic
acid to distilled/deionized water and bring volume to 10.0mL. Mix
thoroughly. Filter sterilize.
Preparation of Medium: Add components, except mevalonic acid
solution and ethanol, to distilled/deionized water and bring volume to
950.0mL. Mix thoroughly. Autoclave for 15 min at 15 psi pressure–
121°C. Aseptically add 40.0mL of filter-sterilized ethanol and 10.0mL
of sterile mevalonic acid solution. Mix thoroughly. Aseptically distrib-
ute into sterile tubes or flasks.
Use: For the cultivation of Lactobacillus homohiochii.

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Lactobacillus Medium