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Lactobacillus Medium II (DSMZ Medium 93)
[所属分类:培养基配方] [发布时间:2021-4-27] [发布人:李佳颐] [阅读次数:] [返回]
Lactobacillus Medium II
(DSMZ Medium 93)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Na-acetate...................................................................................20.0g
Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
Meat extract..................................................................................2.0g
KH 2 PO 4 .........................................................................................0.5g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
DL -Mevalonic acid...................................................................30.0mg
FeSO 4 ·7H 2 O.............................................................................10.0mg
MnSO 4 ·2H 2 O.............................................................................7.5mg
Ethanol.....................................................................................40.0mL
Tween™ 80................................................................................1.0mL
pH 5.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Lactobacillus fructivorans
and Lactobacillus homohiochii
(DSMZ Medium 93)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Glucose.......................................................................................20.0gNa-acetate...................................................................................20.0g
Agar............................................................................................15.0g
Pancreatic digest of casein..........................................................10.0g
Yeast extract..................................................................................5.0g
Meat extract..................................................................................2.0g
KH 2 PO 4 .........................................................................................0.5g
K 2 HPO 4 .........................................................................................0.5g
MgSO 4 ·7H 2 O................................................................................0.2g
DL -Mevalonic acid...................................................................30.0mg
FeSO 4 ·7H 2 O.............................................................................10.0mg
MnSO 4 ·2H 2 O.............................................................................7.5mg
Ethanol.....................................................................................40.0mL
Tween™ 80................................................................................1.0mL
pH 5.2 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat and bring
to boiling. Distribute into tubes or flasks. Autoclave for 15 min at 15
psi pressure–121°C. Pour into sterile Petri dishes or leave in tubes.
Use: For the cultivation and maintenance of Lactobacillus fructivorans
and Lactobacillus homohiochii
