Lash Serum Medium

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lash Serum Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Casamino acids...........................................................................14.0g
NaCl..............................................................................................6.0g
Glucose.........................................................................................2.0g
Maltose .........................................................................................1.5g
Sodium lactate (60% solution)......................................................0.5g
KCl................................................................................................0.1g
CaCl 2 ·2H 2 O ..................................................................................0.1g
Serum solution.......................................................................500.0mL
pH 5.8 ± 0.2 at 25°C
Serum Solution:
Composition per 500.0mL:
NaHCO 3 ........................................................................................0.1g
Bovine serum.........................................................................200.0mL
Preparation of Serum Solution: Add components to distilled/de-
ionized water and bring volume to 500.0mL. Mix thoroughly. Filter
sterilize.
Preparation of Medium: Add components, except serum solution,
to distilled/deionized water and bring volume to 500.0mL. Mix thor-
oughly. Distribute into tubes in 5.0mL volumes. Autoclave for 15 min
at 15 psi pressure–121°C. Cool to 25°C. Aseptically add 5.0mL of ster-
ile serum solution to each tube. Mix thoroughly.
Use: For the cultivation of Trichomonas vaginalis from clinical spec-
imens.