Lasseur Medium (LMG 170)

[所属分类:培养基配方] [发布时间:2021-4-28] [发布人:李佳颐] [阅读次数:] [返回]

Lasseur Medium
(LMG 170)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Glycerol ......................................................................................25.0g
Agar............................................................................................15.0g
L -Asparagine.................................................................................9.0g
MgSO 4 ·7H 2 O................................................................................5.0g
K 2 HPO 4 .........................................................................................2.5g
CaCl 2 ·2H 2 O ................................................................................0.54g
FeSO 4 ·7H 2 O..................................................................................0.1g

pH 6.7 ± 0.2 at 25°C

Preparation of Medium: Add K 2 HPO 4  to 20.0mL of distilled/de-
ionized water. Mix thoroughly. Add remaining components. Bring vol-
ume to 1.0L with distilled/deionized water. Gently heat and bring to
boiling. Adjust pH to 6.7. Distribute into tubes or flasks. Autoclave for
15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or leave
in tubes.
Use: For the cultivation and maintenance of Pseudomonas fluore-
scens.