Mannitol Egg Yolk Polymyxin Agar

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Egg Yolk Polymyxin Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Agar ............................................................................................15.0g
D- Mannitol...................................................................................10.0g
Peptone........................................................................................10.0g
NaCl............................................................................................10.0g
Beef extract...................................................................................1.0g
Phenol Red................................................................................0.025g
Egg yolk emulsion, 50%..........................................................50.0mL
Polymyxin B solution ..............................................................10.0mL
pH 7.1 ± 0.1 at 25°C
Source: Available as a prepared medium from BD Diagnostic Sys-
tems.
Egg Yolk Emulsion, 50%:
Composition per 100.0mL:
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
NaCl (0.9% solution)...............................................................80.0mL
Preparation of Egg Yolk Emulsion, 50%: Soak eggs with 1:100
dilution of saturated mercuric chloride solution for 1 min. Crack eggs
and separate yolks from whites. Mix egg yolks with 1 chicken egg.
Beat to form emulsion. Measure 50.0mL of egg yolk emulsion and add
to 50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize.
Warm to 45°–50°C.
Polymyxin B Solution:
Composition per 10.0mL:
Polymyxin B........................................................................100,000U
Preparation of Polymyxin B Solution: Add polymyxin B to dis-
tilled/deionized water and bring volume to 10.0mL. Mix thoroughly.
Filter sterilize.
Preparation of Medium: Add components—except egg yolk
emulsion, 50%, and polymyxin B solution—to distilled/deionized wa-
ter and bring volume to 940.0mL. Mix thoroughly. Gently heat and
bring to boiling. Autoclave for 15 min at 15 psi pressure–121°C. Cool
to 45°–50°C. Aseptically add 50.0mL of sterile egg yolk emulsion,
50%, and 10.0mL of sterile polymyxin B solution. Mix thoroughly.
Pour into sterile Petri dishes.
Use: For the cultivation and enumeration of Bacillus cereus from
foods.