Mannitol Maltose Agar

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Maltose Agar

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................20.0g
Agar ............................................................................................13.0g
D- Mannitol...................................................................................10.0g
Maltose........................................................................................10.0g
Beef extract...................................................................................5.0g
Papaic digest of soybean meal......................................................5.0g
Polypeptone™...............................................................................5.0g
Dye stock solution, 1000X.........................................................1.0mL
pH 7.8 ± 0.2 at 25°C
Dye Stock Solution, 1000X:
Composition per 100.0mL:
Bromthymol Blue .........................................................................4.0g
Cresol Red.....................................................................................4.0g

Ethanol, 95%..........................................................................100.0mL

Preparation of Dye Stock Solution, 1000X: Add Bromthymol
Blue and Cresol Red to 100.0mL of ethanol. Mix thoroughly.
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Adjust to pH 7.8.
Gently heat and bring to boiling. Distribute into tubes or flasks. Auto-
clave for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes
or leave in tubes.
Use: For the cultivation of Vibrio species from foods.