Mannitol Salt Agar (BAM M97)

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Salt Agar
(BAM M97)

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................75.0g
Agar............................................................................................15.0g
D- Mannitol ..................................................................................10.0g
Polypeptone ................................................................................10.0g
Beef extract...................................................................................1.0g
Phenol Red................................................................................0.025g
pH 7.4 ± 0.2 at 25°C
Preparation of Medium: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly. Gently heat while
stirring and bring to boiling. Distribute into tubes or flasks. Autoclave
for 15 min at 15 psi pressure–121°C. Pour into sterile Petri dishes or
leave in tubes.
Use: For the selective isolation, cultivation, and enumeration of staph-
ylococci. Mannitol-utilizing organisms turn the medium yellow.