Mannitol Salt Agar with Egg Yolk Emulsion

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Salt Agar with Egg Yolk Emulsion

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

NaCl............................................................................................75.0g
Agar ............................................................................................15.0g
D -Mannitol..................................................................................10.0g
Proteose peptone.........................................................................10.0g
Beef extract...................................................................................1.0g
Phenol Red................................................................................0.025g
Egg yolk emulsion.................................................................100.0mL
pH 7.4 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Egg Yolk Emulsion
Composition per 100.0mL:
Chicken egg yolks............................................................................11
Whole chicken egg.............................................................................1
NaCl (0.9% solution)...............................................................50.0mL
Preparation of Egg Yolk Emulsion: Soak eggs with 1:100 dilu-
tion of saturated mercuric chloride solution for 1 min. Crack eggs and
separate yolks from whites. Mix egg yolks with 1 chicken egg. Beat to
form emulsion. Measure 50.0mL of egg yolk emulsion and add to
50.0mL of 0.9% NaCl solution. Mix thoroughly. Filter sterilize. Warm
to 45°–50°C.
Preparation of Medium: Add components, except egg yolk emul-
sion, to distilled/deionized water and bring volume to 900.0mL. Mix
thoroughly. Autoclave for 15 min at 15 psi pressure–121°C. Cool to
50°C. Aseptically add egg yolk emulsion. Mix thoroughly. Pour into
Petri dishes or aseptically distribute into sterile tubes or flasks.
Use: For the selective isolation of pathogenic staphylococci.