Mannitol Selenite Broth with Brilliant Green

[所属分类:培养基配方] [发布时间:2021-5-14] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Selenite Broth with Brilliant Green

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per liter:

Meat peptone.................................................................................5.0g
Yeast extract..................................................................................5.0g
Mannitol........................................................................................5.0g
K 2 HPO 4 .......................................................................................4.35g
KH 2 PO 4 .........................................................................................3.4g
Sodium taurocholate.....................................................................1.0g
Brilliant Green ..........................................................................0.005g
Na 2 SeO 3 ·5H 2 O..............................................................................1.0g
pH 7.0 ± 0.2 at 25°C
Source: This medium is available from HiMedia.
Caution: Sodium hydrogen selenite (sodium biselenite) is a very tox-
ic, corrosive agent and causes teratogenicity; iit should be handled with
great care. If there is contact, wash immediately with lots of water.
Preparation of Medium: Add NaHSeO 3 to distilled/deionized wa-
ter and bring volume to 1.0L. Mix thoroughly. Add remaining compo-
nents. Mix thoroughly. Distribute into tubes or flasks. Gently heat and
bring to boiling. Sterilize in a boiling water bath or free flowing steam
for 10 min. Do not autoclave. Discard the prepared medium if a large
amount of selenite is reduced (indicated by red precipitate at the bot-
tom of the tube).
Use: For the enrichment of Salmonella spp. from feces, foodstuffs,
and other materials.